PSR

One hundred two patients with recurrent and/or metastatic head and neck squamous cell cancer were entered into four consecutive phase II trials, all cisplatinum (C-DDP, 100 mg/m2/cycle)-based. The two combinations tried were C-DDP, bleomycin, and fluorouracil (CFB) on 54 patients, and cisplatinum and vindesin in 36 patients (CV). The CFB combination was given with C-DDP by continuous infusion over 96 hours (23 patients) or on day 1 (31 patients). The CV regimen was also given in two different schedules, with VDS at 3 mg/m2/g weekly (12 patients) or by a 96-hour continuous infusion (0.6 to 1.0...

One hundred two patients with recurrent and/or metastatic head and neck squamous cell cancer were entered into four consecutive phase II trials, all cisplatinum (C-DDP, 100 mg/m2/cycle)-based. The two combinations tried were C-DDP, bleomycin, and fluorouracil (CFB) on 54 patients, and cisplatinum and vindesin in 36 patients (CV). The CFB combination was given with C-DDP by continuous infusion over 96 hours (23 patients) or on day 1 (31 patients). The CV regimen was also given in two different schedules, with VDS at 3 mg/m2/g weekly (12 patients) or by a 96-hour continuous infusion (0.6 to 1.0...

Good management of disposable and reusable supplies may improve surgical efficiency in the operating room (OR) and also corresponds to the best eco-responsible approach. The purpose of this study was to assess the impact of a clinical pharmacist's intervention in the OR on the non-compliant use of medical devices. We also assessed the economic impact of the pharmaceutical intervention. We conducted a monocentric prospective study in the OR of a University hospital over one year. Three surgical specialties: urologic, digestive and gynecologic were audited after a preparatory phase to optimize u...

Good management of disposable and reusable supplies may improve surgical efficiency in the operating room (OR) and also corresponds to the best eco-responsible approach. The purpose of this study was to assess the impact of a clinical pharmacist's intervention in the OR on the non-compliant use of medical devices. We also assessed the economic impact of the pharmaceutical intervention. We conducted a monocentric prospective study in the OR of a University hospital over one year. Three surgical specialties: urologic, digestive and gynecologic were audited after a preparatory phase to optimize u...

Good management of disposable and reusable supplies may improve surgical efficiency in the operating room (OR) and also corresponds to the best eco-responsible approach. The purpose of this study was to assess the impact of a clinical pharmacist's intervention in the OR on the non-compliant use of medical devices. We also assessed the economic impact of the pharmaceutical intervention. We conducted a monocentric prospective study in the OR of a University hospital over one year. Three surgical specialties: urologic, digestive and gynecologic were audited after a preparatory phase to optimize u...

Good management of disposable and reusable supplies may improve surgical efficiency in the operating room (OR) and also corresponds to the best eco-responsible approach. The purpose of this study was to assess the impact of a clinical pharmacist's intervention in the OR on the non-compliant use of medical devices. We also assessed the economic impact of the pharmaceutical intervention. We conducted a monocentric prospective study in the OR of a University hospital over one year. Three surgical specialties: urologic, digestive and gynecologic were audited after a preparatory phase to optimize u...

Good management of disposable and reusable supplies may improve surgical efficiency in the operating room (OR) and also corresponds to the best eco-responsible approach. The purpose of this study was to assess the impact of a clinical pharmacist's intervention in the OR on the non-compliant use of medical devices. We also assessed the economic impact of the pharmaceutical intervention. We conducted a monocentric prospective study in the OR of a University hospital over one year. Three surgical specialties: urologic, digestive and gynecologic were audited after a preparatory phase to optimize u...

Good management of disposable and reusable supplies may improve surgical efficiency in the operating room (OR) and also corresponds to the best eco-responsible approach. The purpose of this study was to assess the impact of a clinical pharmacist's intervention in the OR on the non-compliant use of medical devices. We also assessed the economic impact of the pharmaceutical intervention. We conducted a monocentric prospective study in the OR of a University hospital over one year. Three surgical specialties: urologic, digestive and gynecologic were audited after a preparatory phase to optimize u...

Good management of disposable and reusable supplies may improve surgical efficiency in the operating room (OR) and also corresponds to the best eco-responsible approach. The purpose of this study was to assess the impact of a clinical pharmacist's intervention in the OR on the non-compliant use of medical devices. We also assessed the economic impact of the pharmaceutical intervention. We conducted a monocentric prospective study in the OR of a University hospital over one year. Three surgical specialties: urologic, digestive and gynecologic were audited after a preparatory phase to optimize u...

Good management of disposable and reusable supplies may improve surgical efficiency in the operating room (OR) and also corresponds to the best eco-responsible approach. The purpose of this study was to assess the impact of a clinical pharmacist's intervention in the OR on the non-compliant use of medical devices. We also assessed the economic impact of the pharmaceutical intervention. We conducted a monocentric prospective study in the OR of a University hospital over one year. Three surgical specialties: urologic, digestive and gynecologic were audited after a preparatory phase to optimize u...

Good management of disposable and reusable supplies may improve surgical efficiency in the operating room (OR) and also corresponds to the best eco-responsible approach. The purpose of this study was to assess the impact of a clinical pharmacist's intervention in the OR on the non-compliant use of medical devices. We also assessed the economic impact of the pharmaceutical intervention. We conducted a monocentric prospective study in the OR of a University hospital over one year. Three surgical specialties: urologic, digestive and gynecologic were audited after a preparatory phase to optimize u...

Good management of disposable and reusable supplies may improve surgical efficiency in the operating room (OR) and also corresponds to the best eco-responsible approach. The purpose of this study was to assess the impact of a clinical pharmacist's intervention in the OR on the non-compliant use of medical devices. We also assessed the economic impact of the pharmaceutical intervention. We conducted a monocentric prospective study in the OR of a University hospital over one year. Three surgical specialties: urologic, digestive and gynecologic were audited after a preparatory phase to optimize u...

In adult acute myeloid leukemia (AML), the weight of the contribution of the combined activity of Pgp and MRP1 to drug resistance is not known. To address this question, we compared the activity of these proteins to the in vitro resistance to daunorubicin (DNR), etoposide, and cytosine arabinoside (Ara-C), using the calcein-AM uptake and the 3-[4, 5-di-methyl-thiazol-2, 5-diphenyl] tetrazolium bromide (MTT) assay in 80 adult AML patients. We found no correlation or only a weak correlation between the in vitro drug resistance to DNR and etoposide and MRP1 or Pgp expression or function when test...

In adult acute myeloid leukemia (AML), the weight of the contribution of the combined activity of Pgp and MRP1 to drug resistance is not known. To address this question, we compared the activity of these proteins to the in vitro resistance to daunorubicin (DNR), etoposide, and cytosine arabinoside (Ara-C), using the calcein-AM uptake and the 3-[4, 5-di-methyl-thiazol-2, 5-diphenyl] tetrazolium bromide (MTT) assay in 80 adult AML patients. We found no correlation or only a weak correlation between the in vitro drug resistance to DNR and etoposide and MRP1 or Pgp expression or function when test...

In adult acute myeloid leukemia (AML), the weight of the contribution of the combined activity of Pgp and MRP1 to drug resistance is not known. To address this question, we compared the activity of these proteins to the in vitro resistance to daunorubicin (DNR), etoposide, and cytosine arabinoside (Ara-C), using the calcein-AM uptake and the 3-[4, 5-di-methyl-thiazol-2, 5-diphenyl] tetrazolium bromide (MTT) assay in 80 adult AML patients. We found no correlation or only a weak correlation between the in vitro drug resistance to DNR and etoposide and MRP1 or Pgp expression or function when test...

In adult acute myeloid leukemia (AML), the weight of the contribution of the combined activity of Pgp and MRP1 to drug resistance is not known. To address this question, we compared the activity of these proteins to the in vitro resistance to daunorubicin (DNR), etoposide, and cytosine arabinoside (Ara-C), using the calcein-AM uptake and the 3-[4, 5-di-methyl-thiazol-2, 5-diphenyl] tetrazolium bromide (MTT) assay in 80 adult AML patients. We found no correlation or only a weak correlation between the in vitro drug resistance to DNR and etoposide and MRP1 or Pgp expression or function when test...

In adult acute myeloid leukemia (AML), the weight of the contribution of the combined activity of Pgp and MRP1 to drug resistance is not known. To address this question, we compared the activity of these proteins to the in vitro resistance to daunorubicin (DNR), etoposide, and cytosine arabinoside (Ara-C), using the calcein-AM uptake and the 3-[4, 5-di-methyl-thiazol-2, 5-diphenyl] tetrazolium bromide (MTT) assay in 80 adult AML patients. We found no correlation or only a weak correlation between the in vitro drug resistance to DNR and etoposide and MRP1 or Pgp expression or function when test...

Français À l'aide d'un test immuno-enzymatique indirect anti-dengue à base d'immunoglobulines G (IgG), la séroprévalence a été déterminée chez 783 donneurs de sang adultes dans les îles françaises des Caraïbes de Guadeloupe et de Martinique en 2011. Au total, 93,5 % [91,5 ; 95,1] des échantillons étaient positifs aux anticorps de la dengue, 90,7 % (350 sur 386) en Martinique et 96,2 % (382 sur 397) en Guadeloupe. Seulement 30 % de ces adultes se souvenaient avoir déjà eu la dengue. Des tests de neutralisation spécifiques au sérotype appliqués à un sous-ensemble d'échantillons IgG positifs ont...

Français À l'aide d'un test immuno-enzymatique indirect anti-dengue à base d'immunoglobulines G (IgG), la séroprévalence a été déterminée chez 783 donneurs de sang adultes dans les îles françaises des Caraïbes de Guadeloupe et de Martinique en 2011. Au total, 93,5 % [91,5 ; 95,1] des échantillons étaient positifs aux anticorps de la dengue, 90,7 % (350 sur 386) en Martinique et 96,2 % (382 sur 397) en Guadeloupe. Seulement 30 % de ces adultes se souvenaient avoir déjà eu la dengue. Des tests de neutralisation spécifiques au sérotype appliqués à un sous-ensemble d'échantillons IgG positifs ont...

Français À l'aide d'un test immuno-enzymatique indirect anti-dengue à base d'immunoglobulines G (IgG), la séroprévalence a été déterminée chez 783 donneurs de sang adultes dans les îles françaises des Caraïbes de Guadeloupe et de Martinique en 2011. Au total, 93,5 % [91,5 ; 95,1] des échantillons étaient positifs aux anticorps de la dengue, 90,7 % (350 sur 386) en Martinique et 96,2 % (382 sur 397) en Guadeloupe. Seulement 30 % de ces adultes se souvenaient avoir déjà eu la dengue. Des tests de neutralisation spécifiques au sérotype appliqués à un sous-ensemble d'échantillons IgG positifs ont...

Français À l'aide d'un test immuno-enzymatique indirect anti-dengue à base d'immunoglobulines G (IgG), la séroprévalence a été déterminée chez 783 donneurs de sang adultes dans les îles françaises des Caraïbes de Guadeloupe et de Martinique en 2011. Au total, 93,5 % [91,5 ; 95,1] des échantillons étaient positifs aux anticorps de la dengue, 90,7 % (350 sur 386) en Martinique et 96,2 % (382 sur 397) en Guadeloupe. Seulement 30 % de ces adultes se souvenaient avoir déjà eu la dengue. Des tests de neutralisation spécifiques au sérotype appliqués à un sous-ensemble d'échantillons IgG positifs ont...

Français À l'aide d'un test immuno-enzymatique indirect anti-dengue à base d'immunoglobulines G (IgG), la séroprévalence a été déterminée chez 783 donneurs de sang adultes dans les îles françaises des Caraïbes de Guadeloupe et de Martinique en 2011. Au total, 93,5 % [91,5 ; 95,1] des échantillons étaient positifs aux anticorps de la dengue, 90,7 % (350 sur 386) en Martinique et 96,2 % (382 sur 397) en Guadeloupe. Seulement 30 % de ces adultes se souvenaient avoir déjà eu la dengue. Des tests de neutralisation spécifiques au sérotype appliqués à un sous-ensemble d'échantillons IgG positifs ont...

Français À l'aide d'un test immuno-enzymatique indirect anti-dengue à base d'immunoglobulines G (IgG), la séroprévalence a été déterminée chez 783 donneurs de sang adultes dans les îles françaises des Caraïbes de Guadeloupe et de Martinique en 2011. Au total, 93,5 % [91,5 ; 95,1] des échantillons étaient positifs aux anticorps de la dengue, 90,7 % (350 sur 386) en Martinique et 96,2 % (382 sur 397) en Guadeloupe. Seulement 30 % de ces adultes se souvenaient avoir déjà eu la dengue. Des tests de neutralisation spécifiques au sérotype appliqués à un sous-ensemble d'échantillons IgG positifs ont...

Français À l'aide d'un test immuno-enzymatique indirect anti-dengue à base d'immunoglobulines G (IgG), la séroprévalence a été déterminée chez 783 donneurs de sang adultes dans les îles françaises des Caraïbes de Guadeloupe et de Martinique en 2011. Au total, 93,5 % [91,5 ; 95,1] des échantillons étaient positifs aux anticorps de la dengue, 90,7 % (350 sur 386) en Martinique et 96,2 % (382 sur 397) en Guadeloupe. Seulement 30 % de ces adultes se souvenaient avoir déjà eu la dengue. Des tests de neutralisation spécifiques au sérotype appliqués à un sous-ensemble d'échantillons IgG positifs ont...

Français À l'aide d'un test immuno-enzymatique indirect anti-dengue à base d'immunoglobulines G (IgG), la séroprévalence a été déterminée chez 783 donneurs de sang adultes dans les îles françaises des Caraïbes de Guadeloupe et de Martinique en 2011. Au total, 93,5 % [91,5 ; 95,1] des échantillons étaient positifs aux anticorps de la dengue, 90,7 % (350 sur 386) en Martinique et 96,2 % (382 sur 397) en Guadeloupe. Seulement 30 % de ces adultes se souvenaient avoir déjà eu la dengue. Des tests de neutralisation spécifiques au sérotype appliqués à un sous-ensemble d'échantillons IgG positifs ont...

AbstractThirteen cell lines with different levels of Pgp and MRP expression were used to assess the ability of calcein acetoxymethyl ester (calcein-AM) uptake and calcein efflux to measure Pgp and MRP functions, respectively. There was a good correlation between MRP expression and the modulatory effect of probenecid (a specific modulator of MRP) on the calcein efflux (r = .91, P= .0003) and between Pgp expression and the modulatory effect of CsA on calcein-AM uptake (r = .96, P < .0001). In light of the high correlations for both proteins, we tested calcein-AM uptake and efflux in fresh...

AbstractThirteen cell lines with different levels of Pgp and MRP expression were used to assess the ability of calcein acetoxymethyl ester (calcein-AM) uptake and calcein efflux to measure Pgp and MRP functions, respectively. There was a good correlation between MRP expression and the modulatory effect of probenecid (a specific modulator of MRP) on the calcein efflux (r = .91, P= .0003) and between Pgp expression and the modulatory effect of CsA on calcein-AM uptake (r = .96, P < .0001). In light of the high correlations for both proteins, we tested calcein-AM uptake and efflux in fresh...

AbstractThirteen cell lines with different levels of Pgp and MRP expression were used to assess the ability of calcein acetoxymethyl ester (calcein-AM) uptake and calcein efflux to measure Pgp and MRP functions, respectively. There was a good correlation between MRP expression and the modulatory effect of probenecid (a specific modulator of MRP) on the calcein efflux (r = .91, P= .0003) and between Pgp expression and the modulatory effect of CsA on calcein-AM uptake (r = .96, P < .0001). In light of the high correlations for both proteins, we tested calcein-AM uptake and efflux in fresh...

AbstractThirteen cell lines with different levels of Pgp and MRP expression were used to assess the ability of calcein acetoxymethyl ester (calcein-AM) uptake and calcein efflux to measure Pgp and MRP functions, respectively. There was a good correlation between MRP expression and the modulatory effect of probenecid (a specific modulator of MRP) on the calcein efflux (r = .91, P= .0003) and between Pgp expression and the modulatory effect of CsA on calcein-AM uptake (r = .96, P < .0001). In light of the high correlations for both proteins, we tested calcein-AM uptake and efflux in fresh...

AbstractThirteen cell lines with different levels of Pgp and MRP expression were used to assess the ability of calcein acetoxymethyl ester (calcein-AM) uptake and calcein efflux to measure Pgp and MRP functions, respectively. There was a good correlation between MRP expression and the modulatory effect of probenecid (a specific modulator of MRP) on the calcein efflux (r = .91, P= .0003) and between Pgp expression and the modulatory effect of CsA on calcein-AM uptake (r = .96, P < .0001). In light of the high correlations for both proteins, we tested calcein-AM uptake and efflux in fresh...

Multidrug resistance (MDR) associated with overexpression of the MDR1 gene and of its product, P-glycoprotein (Pgp), plays an important role in limiting cancer treatment efficacy. Many studies have investigated Pgp expression in clinical samples of hematological malignancies but failed to give definitive conclusion on its usefulness. One convenient method for fluorescent detection of Pgp in malignant cells is flow cytometry which however gives variable results from a laboratory to another one, partly due to the lack of a reference method rigorously tested. The purpose of this technical note is...

Multidrug resistance (MDR) associated with overexpression of the MDR1 gene and of its product, P-glycoprotein (Pgp), plays an important role in limiting cancer treatment efficacy. Many studies have investigated Pgp expression in clinical samples of hematological malignancies but failed to give definitive conclusion on its usefulness. One convenient method for fluorescent detection of Pgp in malignant cells is flow cytometry which however gives variable results from a laboratory to another one, partly due to the lack of a reference method rigorously tested. The purpose of this technical note is...

Multidrug resistance (MDR) associated with overexpression of the MDR1 gene and of its product, P-glycoprotein (Pgp), plays an important role in limiting cancer treatment efficacy. Many studies have investigated Pgp expression in clinical samples of hematological malignancies but failed to give definitive conclusion on its usefulness. One convenient method for fluorescent detection of Pgp in malignant cells is flow cytometry which however gives variable results from a laboratory to another one, partly due to the lack of a reference method rigorously tested. The purpose of this technical note is...

Multidrug resistance (MDR) associated with overexpression of the MDR1 gene and of its product, P-glycoprotein (Pgp), plays an important role in limiting cancer treatment efficacy. Many studies have investigated Pgp expression in clinical samples of hematological malignancies but failed to give definitive conclusion on its usefulness. One convenient method for fluorescent detection of Pgp in malignant cells is flow cytometry which however gives variable results from a laboratory to another one, partly due to the lack of a reference method rigorously tested. The purpose of this technical note is...

The initial clinical and biological parameters, including clonogenic leukemic cell (CFU-L) assay, were reviewed for their prognostic significance in a cohort of 188 adult patients with newly diagnosed untreated acute myeloid leukemia (AML). Almost all patients received induction therapy with daunorubicin (DNR) and cytarabine (Ara-C) according to the European Organization for Research and Treatment of Cancer (EORTC) AML 5 to AML 9 trials. Bone marrow samples from 116 representative patients were obtained for CFU-L assay with an efficiency percentage of 89.6%; 76 patients had a measurement of th...

The initial clinical and biological parameters, including clonogenic leukemic cell (CFU-L) assay, were reviewed for their prognostic significance in a cohort of 188 adult patients with newly diagnosed untreated acute myeloid leukemia (AML). Almost all patients received induction therapy with daunorubicin (DNR) and cytarabine (Ara-C) according to the European Organization for Research and Treatment of Cancer (EORTC) AML 5 to AML 9 trials. Bone marrow samples from 116 representative patients were obtained for CFU-L assay with an efficiency percentage of 89.6%; 76 patients had a measurement of th...

The initial clinical and biological parameters, including clonogenic leukemic cell (CFU-L) assay, were reviewed for their prognostic significance in a cohort of 188 adult patients with newly diagnosed untreated acute myeloid leukemia (AML). Almost all patients received induction therapy with daunorubicin (DNR) and cytarabine (Ara-C) according to the European Organization for Research and Treatment of Cancer (EORTC) AML 5 to AML 9 trials. Bone marrow samples from 116 representative patients were obtained for CFU-L assay with an efficiency percentage of 89.6%; 76 patients had a measurement of th...

The initial clinical and biological parameters, including clonogenic leukemic cell (CFU-L) assay, were reviewed for their prognostic significance in a cohort of 188 adult patients with newly diagnosed untreated acute myeloid leukemia (AML). Almost all patients received induction therapy with daunorubicin (DNR) and cytarabine (Ara-C) according to the European Organization for Research and Treatment of Cancer (EORTC) AML 5 to AML 9 trials. Bone marrow samples from 116 representative patients were obtained for CFU-L assay with an efficiency percentage of 89.6%; 76 patients had a measurement of th...

The initial clinical and biological parameters, including clonogenic leukemic cell (CFU-L) assay, were reviewed for their prognostic significance in a cohort of 188 adult patients with newly diagnosed untreated acute myeloid leukemia (AML). Almost all patients received induction therapy with daunorubicin (DNR) and cytarabine (Ara-C) according to the European Organization for Research and Treatment of Cancer (EORTC) AML 5 to AML 9 trials. Bone marrow samples from 116 representative patients were obtained for CFU-L assay with an efficiency percentage of 89.6%; 76 patients had a measurement of th...

The initial clinical and biological parameters, including clonogenic leukemic cell (CFU-L) assay, were reviewed for their prognostic significance in a cohort of 188 adult patients with newly diagnosed untreated acute myeloid leukemia (AML). Almost all patients received induction therapy with daunorubicin (DNR) and cytarabine (Ara-C) according to the European Organization for Research and Treatment of Cancer (EORTC) AML 5 to AML 9 trials. Bone marrow samples from 116 representative patients were obtained for CFU-L assay with an efficiency percentage of 89.6%; 76 patients had a measurement of th...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

AbstractWe characterized the localization, phenotype, and some functions of plasmacytoid dendritic cells (pDCs) in the human spleen. pDCs were localized in the marginal zone and the periarteriolar region. Some were also found in the red pulp. pDCs were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from HIV-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDCs, but not myeloid dendritic cells (mDCs), was observed in some HIV+ patients, correlating with...

Abstract Drug resistance is one of the major reasons for failure of chemotherapy of acute leukemia with cytosine arabinoside (ARA‐C). In order to overcome this problem we have investigated the interaction of ARA‐C with 3‐deazauridine (3‐DU) against HL‐60 myeloid leukemic cells. 3‐DU is an interesting agent to use in combination with ARA‐C, since drug‐resistant cells that are deficient in deoxycytidine kinase are very sensitive to this uridine analogue. We have observed that for both short and long drug exposure there was a potent synergistic interaction between ARA‐C and 3‐DU with respect to t...

Abstract Drug resistance is one of the major reasons for failure of chemotherapy of acute leukemia with cytosine arabinoside (ARA‐C). In order to overcome this problem we have investigated the interaction of ARA‐C with 3‐deazauridine (3‐DU) against HL‐60 myeloid leukemic cells. 3‐DU is an interesting agent to use in combination with ARA‐C, since drug‐resistant cells that are deficient in deoxycytidine kinase are very sensitive to this uridine analogue. We have observed that for both short and long drug exposure there was a potent synergistic interaction between ARA‐C and 3‐DU with respect to t...

Abstract Drug resistance is one of the major reasons for failure of chemotherapy of acute leukemia with cytosine arabinoside (ARA‐C). In order to overcome this problem we have investigated the interaction of ARA‐C with 3‐deazauridine (3‐DU) against HL‐60 myeloid leukemic cells. 3‐DU is an interesting agent to use in combination with ARA‐C, since drug‐resistant cells that are deficient in deoxycytidine kinase are very sensitive to this uridine analogue. We have observed that for both short and long drug exposure there was a potent synergistic interaction between ARA‐C and 3‐DU with respect to t...

Abstract Drug resistance is one of the major reasons for failure of chemotherapy of acute leukemia with cytosine arabinoside (ARA‐C). In order to overcome this problem we have investigated the interaction of ARA‐C with 3‐deazauridine (3‐DU) against HL‐60 myeloid leukemic cells. 3‐DU is an interesting agent to use in combination with ARA‐C, since drug‐resistant cells that are deficient in deoxycytidine kinase are very sensitive to this uridine analogue. We have observed that for both short and long drug exposure there was a potent synergistic interaction between ARA‐C and 3‐DU with respect to t...

Abstract Drug resistance is one of the major reasons for failure of chemotherapy of acute leukemia with cytosine arabinoside (ARA‐C). In order to overcome this problem we have investigated the interaction of ARA‐C with 3‐deazauridine (3‐DU) against HL‐60 myeloid leukemic cells. 3‐DU is an interesting agent to use in combination with ARA‐C, since drug‐resistant cells that are deficient in deoxycytidine kinase are very sensitive to this uridine analogue. We have observed that for both short and long drug exposure there was a potent synergistic interaction between ARA‐C and 3‐DU with respect to t...

Abstract Drug resistance is one of the major reasons for failure of chemotherapy of acute leukemia with cytosine arabinoside (ARA‐C). In order to overcome this problem we have investigated the interaction of ARA‐C with 3‐deazauridine (3‐DU) against HL‐60 myeloid leukemic cells. 3‐DU is an interesting agent to use in combination with ARA‐C, since drug‐resistant cells that are deficient in deoxycytidine kinase are very sensitive to this uridine analogue. We have observed that for both short and long drug exposure there was a potent synergistic interaction between ARA‐C and 3‐DU with respect to t...

Abstract Drug resistance is one of the major reasons for failure of chemotherapy of acute leukemia with cytosine arabinoside (ARA‐C). In order to overcome this problem we have investigated the interaction of ARA‐C with 3‐deazauridine (3‐DU) against HL‐60 myeloid leukemic cells. 3‐DU is an interesting agent to use in combination with ARA‐C, since drug‐resistant cells that are deficient in deoxycytidine kinase are very sensitive to this uridine analogue. We have observed that for both short and long drug exposure there was a potent synergistic interaction between ARA‐C and 3‐DU with respect to t...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

Background and Objectives. In the EORTC ALL-3 trial, the efficacy of allogeneic trans plantation was compared with that of autologous marrow transplantation and main tenance chemotherapy in patients ≤ 50 years who reached CR. Design and Methods. Among 340 patients who entered the study, 279 were ≤ 50 years old. Out of these, 220 reached CR, 184 patients started consolidation and were HLA typed; 68 had a donor and 116 had no sibling donor. The median follow-up was 9.5 years; 93 patients relapsed, 26 died in CR, and overall 116 patients died. Allogeneic transplantation was performed...

La présente étude a consisté à tester l’efficacité des extraits de feuilles de papayer (Carica papaya) et des extraits de feuilles et de graines de neem (Azadirachta indica) sur le gombo (Abelmoschus esculentus) et le chou (Brassica oleracea). Neuf traitements ont été comparés : un témoin non traité, un témoin traité avec un insecticide homologué à base de cartap, six traitements avec trois différentes doses d’extraits de neem et de papayer et un traitement avec un extrait de graines de neem. L’extrait de graines de neem a été très efficace, tout comme le produit chimique, contre les chenilles...

La présente étude a consisté à tester l’efficacité des extraits de feuilles de papayer (Carica papaya) et des extraits de feuilles et de graines de neem (Azadirachta indica) sur le gombo (Abelmoschus esculentus) et le chou (Brassica oleracea). Neuf traitements ont été comparés : un témoin non traité, un témoin traité avec un insecticide homologué à base de cartap, six traitements avec trois différentes doses d’extraits de neem et de papayer et un traitement avec un extrait de graines de neem. L’extrait de graines de neem a été très efficace, tout comme le produit chimique, contre les chenilles...

La présente étude a consisté à tester l’efficacité des extraits de feuilles de papayer (Carica papaya) et des extraits de feuilles et de graines de neem (Azadirachta indica) sur le gombo (Abelmoschus esculentus) et le chou (Brassica oleracea). Neuf traitements ont été comparés : un témoin non traité, un témoin traité avec un insecticide homologué à base de cartap, six traitements avec trois différentes doses d’extraits de neem et de papayer et un traitement avec un extrait de graines de neem. L’extrait de graines de neem a été très efficace, tout comme le produit chimique, contre les chenilles...

La présente étude a consisté à tester l’efficacité des extraits de feuilles de papayer (Carica papaya) et des extraits de feuilles et de graines de neem (Azadirachta indica) sur le gombo (Abelmoschus esculentus) et le chou (Brassica oleracea). Neuf traitements ont été comparés : un témoin non traité, un témoin traité avec un insecticide homologué à base de cartap, six traitements avec trois différentes doses d’extraits de neem et de papayer et un traitement avec un extrait de graines de neem. L’extrait de graines de neem a été très efficace, tout comme le produit chimique, contre les chenilles...

La présente étude a consisté à tester l’efficacité des extraits de feuilles de papayer (Carica papaya) et des extraits de feuilles et de graines de neem (Azadirachta indica) sur le gombo (Abelmoschus esculentus) et le chou (Brassica oleracea). Neuf traitements ont été comparés : un témoin non traité, un témoin traité avec un insecticide homologué à base de cartap, six traitements avec trois différentes doses d’extraits de neem et de papayer et un traitement avec un extrait de graines de neem. L’extrait de graines de neem a été très efficace, tout comme le produit chimique, contre les chenilles...

Arrière-plan Les zones humides et les cultures agricoles irriguées constituent des sites de reproduction potentiels pour les moustiques anophèles , ce qui entraîne une hétérogénéité de la transmission du paludisme. En zone agricole, cette hétérogénéité est souvent associée à la présence de foyers localisés de transmission plus intense. Cette étude vise à identifier les foyers microgéographiques de transmission du paludisme en milieu agricole grâce à une approche multidisciplinaire. Méthodes Deux enquêtes transversales ont été menées à la fin de la saison sèche et au plus fort de la saison...

Arrière-plan Les zones humides et les cultures agricoles irriguées constituent des sites de reproduction potentiels pour les moustiques anophèles , ce qui entraîne une hétérogénéité de la transmission du paludisme. En zone agricole, cette hétérogénéité est souvent associée à la présence de foyers localisés de transmission plus intense. Cette étude vise à identifier les foyers microgéographiques de transmission du paludisme en milieu agricole grâce à une approche multidisciplinaire. Méthodes Deux enquêtes transversales ont été menées à la fin de la saison sèche et au plus fort de la saison...

Arrière-plan Les zones humides et les cultures agricoles irriguées constituent des sites de reproduction potentiels pour les moustiques anophèles , ce qui entraîne une hétérogénéité de la transmission du paludisme. En zone agricole, cette hétérogénéité est souvent associée à la présence de foyers localisés de transmission plus intense. Cette étude vise à identifier les foyers microgéographiques de transmission du paludisme en milieu agricole grâce à une approche multidisciplinaire. Méthodes Deux enquêtes transversales ont été menées à la fin de la saison sèche et au plus fort de la saison...

Arrière-plan Les zones humides et les cultures agricoles irriguées constituent des sites de reproduction potentiels pour les moustiques anophèles , ce qui entraîne une hétérogénéité de la transmission du paludisme. En zone agricole, cette hétérogénéité est souvent associée à la présence de foyers localisés de transmission plus intense. Cette étude vise à identifier les foyers microgéographiques de transmission du paludisme en milieu agricole grâce à une approche multidisciplinaire. Méthodes Deux enquêtes transversales ont été menées à la fin de la saison sèche et au plus fort de la saison...

Arrière-plan Les zones humides et les cultures agricoles irriguées constituent des sites de reproduction potentiels pour les moustiques anophèles , ce qui entraîne une hétérogénéité de la transmission du paludisme. En zone agricole, cette hétérogénéité est souvent associée à la présence de foyers localisés de transmission plus intense. Cette étude vise à identifier les foyers microgéographiques de transmission du paludisme en milieu agricole grâce à une approche multidisciplinaire. Méthodes Deux enquêtes transversales ont été menées à la fin de la saison sèche et au plus fort de la saison...